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SELDI EVMS UnProcessed Data

Dataset Abstract:

Unprocessed SELDI bioprocessor output from EVMS (Eastern Virginia Medical School), EDRN Site 92. Each spectra was calibrated and normalized to an external coefficient of 0.2 using Ciphergen's "Peaks" software. Raw time of flight values were then exported as combined spread sheets representing many individual spectra produced from serum samples in each study. These files are comma delimited (csv).


The following additional information has been defined for this dataset. The information has been provided by the Principal Investigator or staff from his or her laboratory.

ProtocolId
110
ProtocolName
SELDI Validation Study Phase II
DataSetName
SELDI EVMS UnProcessed Data
LeadPI
John Semmes
SiteName
University of Washington (Associate Member)
DataCustodian
Dale McLerran
OrganSite
Prostate
CollaborativeGroup
Prostate and Urologic
Date
2007-08-07T15:46:00.000Z
QAState
Accepted
SpecificAims
Specific Aim Phase II - The overall goal of Phase II is to develop and evaluate an algorithm for classifying cases and controls using protein profiles produced from SELDI-TOF-MS using serum collected from prostate cancer cases and non-cancer controls. Objective 1 - Identification of biorepositories Objective 2 - Generate protein profiles Objective 3 - Construction of classifiers and development of algorithm. Objective 4 – Validate the classifier constructed in Objective 3. If Objective 3, Aim 1, is successfully met
PlannedSampleSize
The EDRN-Prostate-SELDI Investigational Collaboration (EPSIC) will implement a process to synchronize SELDI output at the seven participating institutions using a single source of pooled normal sera (QC). The target was to recruit 100 healthy males and 100 healthy females to donate two tubes of blood. Pregnant women and subjects less than 110 pounds were excluded from the blood collection. An IRB protocol was submitted two months in advance of the project. After IRB approval, fliers were generated and posted at many locations (e.g., EVMS, hospitals, doctor's offices). The blood was collected on November 6, 2002 from 7:30 am to 2:00 pm. A total of 360 healthy people (154 male and 206 female) donated two tubes of blood each to make quality control (QC) serum.
StudyObjective
It is then the goal of this collaborative project - EDRN-Prostate-SELDI Investigational Collaboration (EPSIC) - to use state-of-the-art protein profiling technology to develop and validate such screening methods
DataDisclaimer
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