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University of Washington Immunohistochemistry Data

Dataset Abstract:

Data capturing investigation of the molecular basis of cell-cell interaction in the differentiation of epithelial cells of the human bladder. The approach involves cell-type identification by CD cell surface molecules, isolation of specific cell populations, gene expression analysis, and functional test of candidate genes in a cell culture system.


The following additional information has been defined for this dataset. The information has been provided by the Principal Investigator or staff from his or her laboratory.

ProtocolId
201
ProtocolName
Clinical Utility of Urinary CD90 as a Biomarker for Prostate Cancer Detection
StudyDescription
We are investigating the molecular basis of cell-cell interaction in the differentiation of epithelial cells of the human bladder. Our approach involves cell-type identification by CD cell surface molecules, isolation of specific cell populations, gene expression analysis, and functional test of candidate genes in a cell culture system.
StudyBackground
Stromal mesenchyme cells play an important role in epithelial differentiation and likely in cancer as well. Induction of epithelial differentiation is organ-specific, and the genes responsible could be identified through a comparative genomic analysis of the stromal cells from two different organs. These genes might be aberrantly expressed in cancer since cancer could be viewed as due to a defect in stromal signaling. We propose to identify the prostate stromal genes by analysis of differentially expressed genes between prostate and bladder stromal cells, and to examine their expression in prostate cancer.
DataSetName
University of Washington Immunohistochemistry Data
LeadPI
Alvin Liu
SiteName
University of Washington (Biomarker Developmental Laboratories)
DataCustodian
Tina Xiao
DataCustodianEmail
TBD
OrganSite
Prostate
CollaborativeGroup
Prostate and Urologic
MethodDetails
TBD
ResultsAndConclusionSummary
TBD
Date
2008-06-24T20:12:00.000Z
QAState
Accepted
DataCustodianPhone
TBD
ResearchSupport
N.I.H., Extramural
StudyDesign
TBD
StudyConclusion
Our findings show that the histologically similar stromas of the prostate and bladder are phenotypically different, and express organ-specific genes. The importance of these genes in epithelial development is suggested by their abnormal expression in cancer. Among the candidates is the hormone PENK and the down-regulation of PENK expression in cancer suggests a possible association with cancer development.
StudyResults
The bladder stroma was phenotypically different from that of the prostate. Most notable was the presence of a layer of CD13 cells adjacent to the urothelium. This structural feature was also seen in the mouse bladder. The prostate stroma was uniformly CD13-. A number of differentially expressed genes between prostate and bladder stromal cells were identified. One prostate gene, proenkephalin (PENK), was of interest because it encodes a hormone. Secreted proteins such as hormones and bioactive peptides are known to mediate cell-cell signaling. Prostate stromal expression of PENK was verified by an antibody raised against a PENK peptide, by RT-PCR analysis of laser-capture microdissected stromal cells, and by database analysis. Gene expression analysis showed that PENK expression was down-regulated in prostate cancer.
AnalyticMethods
TBD
GrantSupport
CA85859/CA/NCI
CA98699/CA/NCI
DK63630/DK/NIDDK
DK65260/DK/NIDDK
StudyMethods
Immunohistochemistry using antibodies to cluster designation (CD) cell surface antigens was first used to characterize the stromas of the prostate and bladder. Stromal cells were prepared from either prostate or bladder tissue for cell culture. RNA was isolated from the cultured cells and analyzed by DNA microarrays. Expression of candidate genes in normal prostate and prostate cancer was examined by RT-PCR.
EligibilityCriteria
TBD
DataDisclaimer
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