Early Detection Research Network
Team Project

BRSCW Reference Set Application: Karen Abbott -University of Arkansas (2014)

BRSCW Ref Set App: Abbott (2014)
395
Feng, ZidingFred Hutchinson Cancer Research Center
Assay-MagPix instrument using the magnetic spectral beads.
No design specified.
Glycomics
Breast and Gynecologic Cancers Research Group

Our earlier glycoproteomic studies have identified bisecting glycoslyation and core fucosylation changes on particular glycoproteins in endometrioid ovarian cancer tissues and plasma (Abbott et al, 2010, Proteomics). We have validated that these glycan changes occur on the same glycoproteins in serous ovarian cancer plasma using a lectin-pull down western blot assays. We would like to used pooled reference samples to develop a sensitive magnetic bead-based assay to detect these glycoproteins with bisecting and core fucosylation changes.

We would like to used pooled reference samples to develop a sensitive magnetic bead-based assay to detect these glycoproteins with bisecting and core fucosylation changes. We have purified fusion-tagged glycoproteins with the specific glycan structures that we will spike into the control samples to determine the range and variation of detection prior to the phase II sample analysis. We will also measure the variance between the malignant ovarian cancer pooled samples and control benign pooled samples to determine if the assay can measure statistically significant differences.
Data will be analyzed by the DMCC

There are currently no biomarkers annotated for this protocol.

No datasets are currently associated with this protocol.